ot-ii peptide II - Ovapeptide OVA (329-339) induces to reach the half-maximal proliferation Understanding the OT-II Peptide: A Key Player in T Cell Immunology

Ot1 mice The OT-II peptide is a critical element in immunological research, particularly for understanding T cell responses. This specific peptide is derived from ovalbumin (OVA), a well-studied protein found in egg whites.The Peptide Ligands Mediating Positive Selection in the ... The OT-II peptide, more precisely, refers to a specific sequence within ovalbumin that is recognized by a particular T cell receptorVaccine delivery by penetratin: mechanism of antigen .... This recognition is fundamental to studying adaptive immunity, especially the role of CD4+ T cells作者：F Moalli·2014·被引用次数：41—We therefore generated TP−/−OT-IICD4+T cells recognizing the OVA323-339peptide. Despite high expression of TP in thymocytes, TP−/−OT-II....

The core of the OT-II peptide's significance lies in its ability to activate OT-II T cells. These are transgenic T cells that express a T cell receptor (TCR) specifically engineered to recognize the OVA peptide 323-339 fragment when presented by MHC class II molecules, particularly the H-2Ab haplotype. This specificity makes OT-II T cells an invaluable tool in research models. The OVA peptide 323-339 is a well-characterized T and B cell epitope, crucial for generating and developing immune responses, including immediate hypersensitivity. Researchers frequently use this OVA peptide for activation studies, often in conjunction with OVA wt peptide 323-339作者：JM Robertson·2000·被引用次数：478—DO11.10 and OT-II T cells areboth specific for a C-terminal epitopewhose core encompasses amino acids 329-337..

The OT-II T cells are often utilized in transgenic mouse models, such as the OT-II mouse, which allows scientists to track and manipulate antigen-specific T cell behavior in vivo. These mice express the mouse alpha-chain and beta-chain T cell receptor that pairs with the CD4 co-receptor, making them specifically reactive to the OT-II peptide. Various strains, like the Rag2/OT-II mouse model, are developed to facilitate T cell biology research by combining the OT-II transgene with deficiencies in recombination activation gene 2040737 - OT-2 CD45.1 Cas9 Strain Details. Another relevant strain is the OT-2 CD45.Vaccine delivery by penetratin: mechanism of antigen ...1 Cas9 Strain, which carries the OT-2 transgene designed to recognize ovalbumin peptide residues 323-339 and expresses CD45.1.

The interaction between the OT-II peptide and the T cell receptor is a prime example of how Proteins of the class II major histocompatibility complex (MHC) present antigenic peptides to T cells. Specifically, the OT-II peptide is presented by MHC class II molecules, allowing CD4+ T cells to recognize it. This presentation is essential for initiating adaptive immune responses. For instance, IA b OVA 323-339 Tetramer (Code No. TS-M710-1) is a tool that can be used to monitor antigen-specific CD4 T cell responses in OT-II mice and other experimental systems. Studies have shown that MHC class II deprivation impairs CD4 T cell motility and function, highlighting the importance of this presentation pathway.

Research utilizing the OT-II peptide has led to significant insights. For example, it has been demonstrated that OT-II-derived CD4+ T cells recognize OVA peptide at position 323–339 in the context of H-2AbAny cell line that secretes OVA peptide for OTI or OTII?. Furthermore, the OT-II model reveals dual in vitro and in vivo immunomodulatory effects, suggesting the receptor's complex role in T-cell activation.2014年9月14日—I want to activate CD4+ T cells (fromOTIImice) in the presence of IL-27 using OVApeptide. I would like to use a suspension of whole splenocytes to do this. OT-II cells can be activated with the OVA peptide 323-339, and studies investigate various methods for this activation, including using whole splenocytes. Some research even explores vaccine delivery by penetratin: mechanism of antigen presentation and how it affects OT-II cells.

The precise sequence of the OT-II peptide can vary slightly depending on the research context004194 - OT-II Strain Details. While OVA peptide 323-339 is commonly cited, some sources refer to OVA (329-337) or OVA (329-339) as epitopes recognized by OT-II cells.2025年12月12日—OT-2 CD45.1 Cas9 mutant mice express CD45.1 and carry theOT-2 transgene designed to recognize ovalbuminpeptideresidues 323-339 and a ... For instance, a study noted that OVA (329-339) induces to reach the half-maximal proliferation with 25 µM and 30 µM in DO11.10 and OT-II cell, respectively.HLA-A2-restricted synthetic glioma antigen peptides vaccine Similarly, a shorter version of the epitope, OVA tetramer containing a shorter version of the 323-339 epitope, has been shown to result in improved staining of OT-II cells.

In summary, the OT-II peptide is a vital research tool, primarily the OVA peptide 323-339, which serves as a specific antigen for OT-II T cells. This interaction, mediated by MHC class II molecules, is fundamental to understanding T cell immunology and is extensively studied using transgenic OT-II mouse models and related peptides作者：B Ernst·1999·被引用次数：971—OT-IIcells to H2-M2 hosts et al., 1993). would deprive the cells from contact with the ligands that led to positive selection. In this respect, the notable.. The research surrounding the OT-II peptide contributes significantly to our knowledge of immune responses, antigen presentation, and T cell activationThromboxane A2 acts as tonic immunoregulator by ....